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KMID : 0357919740080010015
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Korean Journal of Pathology
1974 Volume.8 No. 1 p.15 ~ p.20
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Micro Serum Glucose Determinations with Neocuproine Hydrochloride
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채범석(蔡範錫)/B.S. Tchai
조경조(曺景燥)/Kyong Hwan Cho
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Abstract
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A simp1e and micro method for the determination of the blood sugar was studied.
serum glucose was estimated by neocuproine hydrochloride method previously described
by M.E. Brown.
Neocuproine method was compared with a classical Somogyi-Nelson method and also
two filtrates namely Somogyi and Folia-Wu were used for the determination of glucose
according to the neocuproine method.
The values of glucose in serum of thirty seven healthy person by classical
Somogyi-Nelson method were 8.60 ±7.5mg/100m1, by Somogyi filtrate-neocuproine
method, 85.8±12.4mg/100ml and by Folia-Wu filtrate-neocuproine method, 102 ±
7.4mg/100m1 respectively.
The estimation of glucose by a Somogyi-Nelson procedure and by the neocuproine is
affected by the protein-free filtrate, and intraprocedual differences are small. However,
consistantly lower values are obtained by the Somogyi-Nelson and Somogyi-neocuproine
methods and provably represent a mere valid index of true serum glucose than the
Folin-Wu neocuproine procedure.
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